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ELISPOT 產品

時間:2017-11-27 12:32:34 瀏覽:

DESCRIPTION:

The ELISPOT assay is a powerful tool for detecting and analyzing individual cells that secrete a particular protein in vitro. Although originally developed for analyzing specific antibody-secreting cells, the assay has been adapted for measuring the frequencies of cells that produce and secrete a variety of other effector molecules such as cytokines.

FEATURES:

-Direct analysis of frequency of cells producing cytokines and other effector molecules using enzyme-linked immunospot (ELISPOT) assay

-Greater sensitivity than ELISA and intracellular staining

-High-throughput analyses for direct ex vivo monitoring of T-cell function, including antigen-specific T cells

BD ELISPOT kits, with pre-coated plates, sets, and pairs are available for human, non-human primate, and mouse research.

BD ELISPOT Reagent Pairs

Sufficient capture antibody and detection antibody for 5 plates.

BD ELISPOT Reagent Sets

Sufficient capture antibody, detection antibody, and streptavidin-horseradish peroxidase for 10 plates worth of assays (includes 10 uncoated plates).

BD ELISPOT Kits with Pre-Coated Plates

Designed for optimal spot quality, maximum time savings, and convenience, this kit includes all necessary reagents.

 

PRODUCT LIST:  

 

 

Size

Status

Cat. No.

 

10 Plate(s)

RUO

551951Quick View

5 Plate(s)

RUO

551881Quick View

5 Plate(s)

RUO

551880Quick View

10 Plate(s)

RUO

552567Quick View

2 Plate(s)

RUO

552569Quick View

10 Plate(s)

RUO

551083Quick View

5 Plate(s)

RUO

557982Quick View

10 Plate(s)

RUO

551076Quick View

5 Plate(s)

RUO

551876Quick View

10 Plate(s)

RUO

551017Quick View

5 Plate(s)

RUO

551878Quick View

10 Plate(s)

RUO

552574Quick View

5 Plate(s)

RUO

551884Quick View

10 Plate(s)

RUO

551084Quick View

10 Plate(s)

RUO

551085Quick View

10 Plate(s)

RUO

552572Quick View

2 Plate(s)

RUO

552138Quick View

10 Plate(s)

RUO

551491Quick View

5 Plate(s)

RUO

551875Quick View

5 Plate(s)

RUO

551882Quick Vi

10 Plate(s)

RUO

551446Quick View

10 Plate(s)

RUO

551075Quick View

10 Plate(s)

RUO

551849Quick View

5 Plate(s)

RUO

551873Quick View

5 Plate(s)

RUO

551883Quick View

10 Plate(s)

RUO

551018Quick View

10 Plate(s)

RUO

551282Quick View

5 Plate(s)

RUO

551885Quick View

5 Plate(s)

RUO

551886Quick Vie

 

BD ELISPOT Assay Procedure:

Capture Antibody (For sets and pairs): Coat microwells with anti-cytokine capture antibody. For Kits: Go to Step 3. Steps 1 and 2 are not necessary. 
Blocking: Block unoccupied sites with protein. 
Add cells: Incubate cells in well with Ag/stimulus, etc. 
Wash: Wash cells. 
Detection Antibody: Add biotinylated anti-cytokine detection antibody. 
Enzyme-Streptavidin: Add streptavidin-horseradish peroxidase. 
Develop with substrate: Add substrate and monitor formation of colored spots.

Features of BD ELISPOT assays

The BD ELISPOT assay is able to detect cytokine release at the single-cell level, allowing for direct determination of cytokine-producing cell frequencies. This assay has been found to be more sensitive than ELISA and intracellular staining. The sensitivity of the assay enables measurement of very low frequencies of cytokine-producing cells (eg, 1 in 300,000).

Recent developments in assay plate design and in high-throughput ELISPOT plate-reader instrumentation have significantly improved the usefulness of the ELISPOT method. Objective and rapid analyses of cytokine-producing cell numbers (spots) and relative amounts of cytokine produced per cell (spot size) are now possible.

BD ELISPOT assays enable high-sensitivity and high-throughput analyses for direct ex vivo monitoring of T-cell function, including antigen-specific T cells.

Quality antibody reagents result in crisp, clear results

BD ELISPOT reagents have been carefully screened to ensure the generation of crisp, clearly-defined immunospots. High-affinity BD Pharmingen? NA/LE capture antibodies safeguard against nonspecific cellular activation. Detection antibodies have been carefully selected, along with pre-optimized preparations of streptavidin-horseradish peroxidase, substrate, and chromogen to produce optimal BD ELISPOT reagents.

Automated coating process ensures consistent results

For the kit format, BD Biosciences - Discovery Labware, the leader in biological coating and an ISO9001 facility, uses state-of-the-art automation to coat the BD ELISPOT plates to guarantee high-quality assay results with minimal well-to-well and plate-to-plate variation. The plates are coated with no azide/low endotoxin capture antibodies, are UV processed, and are tested and found negative for bacteria and fungi.

 

 

 

說明書下載.pdf

 

 

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